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1A) but also in various other tumors such as ESCA, GBM, KIRP, OV, and PAAD (Fig

In brief, cells growing on tissue culture vessels were harvested with cell scraper on ice then lysed with RIPA Lysis Buffer (Millipore) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific) at 4 C for 1 h